Adriana Larregina, MD, PhD

Adriana Larregina, MD, PhD

Contact

Campus: W1051 BST

Ph: 412-383-9842

Fax: 412-648-8117

adrianal@pitt.edu

Education

  • MD - National University of La Plata (1983)
  • PhD - University of Buenos Aires (1996)
  • Post-graduate - Hospital General de Agudos "San Roque" La Plata, Buenos Aires, Argentina (1983-1984)
  • Post-graduate - "CEMIC". University of Buenos Aires

Academic Affiliation

  • Associate Professor, Dept. of Dermatology

About Research

Genetic Vaccines - two sub-projects:

1A) Generation of dendritic cell (DC) vaccines for tumours and infectious diseases: this sub-project uses passive transfer of human and mouse DC transduced in vitro to express transgenic Ag. It explores different factors involved in efficient expression and presentation of transgenic Ag by DC that signalled for efficient activation and immunostimulatory function.
1B) Skin genetic immunization: this sub-project studies expression of transgenic Ag ex-vivo and in vivo of mouse and human skin cells including skin resident DC after transfection with naked plasmid DNA combined with different adjuvants. Selection of adjuvants include: exogenous danger signals (such as Toll Like receptor agonists) and intrinsic danger signals such as pro-inflammatory cytokines and neuropeptides. The project studies their effect on dendritic cell activation and immunostimulatory function and the consequences in the outcome of the immune response.
 

Immuno-dermatology: Skin immunology and dendritic cell biology - two sub-projects:

2A) Study of DC migration out of skin and repopulation by DC precursors
2B) Study of the modulation of specific immune responses targeting skin Ag presenting cells: The main focus of the project is to understand the role of the skin microenvironment in modulating the function of resident DC. It analyzes the immunostimulator and tolerogenic functions of different populations of skin resident dendritic cells including Langerhans cells, dermal dendritic cells and CD14+ dendritic cell precursors.

Selected Publications

Larregina AT, Morelli AE. Interaction between dendritic cells and apoptotic cells. In: “Handbook of dendritic cells- Biology, diseases and therapies”. (Ed. M.B. Lutz, N. Romani, and A. Steinkasserer). Wiley-VCH Press, in press. 2006.

Larregina AT, Falo Jr. LD. Changing paradigms in cutaneous immunology: adapting with dendritic cells. J Invest Dermatol 124:1-12. 2005.

Morelli AE, Rubin P, Tkacheva O, Zahorchack A, Thomson AW, Falo Jr. LD, Larregina AT. Induction of CD4+ T cell responses by different subpopulations human skin dendritic cells”. 175:7905-7915. 2005.

Hokey DA, Larregina AT, Erdos G, Watkins SC, and Falo, Jr. LD. Tumor cell loaded murine type-1 polarized DCs induce Th1-mediated tumor immunity. Cancer Research 65:10059-1006. 2005.

Morelli Adrian, Larregina AT, Schufesky WJ, Sullivan ML, Beer-Stolz D, Papworth G, Zahorchack AF, Logar AJ, Wang Z, Watkins, SC, Falo LD, Thomson AW. Endocytosis, intacellular sorting and processing, of exosomes by dendritic cells. Blood 104:3257-3266. 2004.

Larregina AT, Morelli AE, Tkacheva O, Erdos G, Watkins SC, Thomson AW, Falo Jr. LD. Highly efficient expression of transgenic proteins by naked DNA-transfected dendritic cells through terminal differentiation. Blood103:811-819. 2004.

Morelli AE, Larregina AT, Shufesky WJ, Zahorchak AF, Logar AJ, Papworth GD, Wang Z, Watkins SC, Falo LD Jr, Thomson AW. Internalization of circulating apoptotic cells by splenic marginal zone dendritic cells: dependence on complement receptors and effect on cytokine production. Blood 101:611-620. 2003.

Zimmer MI, Larregina AT, Castillo CM, Capuano III S, Falo Jr. LD, Murphey Corb M, Reinhart TA, Barrat-Boyes SM. Disrupted homeostasis of Langerhans cells and Interdigitant dendritic cells in monkeys with AIDS. Blood 99:2859-2868. 2002.

Larregina AT, Watkins SC, Erdos G, Spencer L, Storkus WJ, Beer Stolz D, Falo LD Jr. Genetic Immunization Through the Skin: Direct Transfection and Activation of Human Cutaneous Dendritic Cells. Gene Ther 8:608-17. 2001.

Larregina AT, Morelli AE, Spencer LA, Logar A, Watkins SC, Thomson AW, Falo LD Jr. Dermal CD14+ cells differentiate into Langerhans cells in the presence of GM-SCF, IL-4 and TGF- 1. Nature Immunology 2:1151-1158. 2001

Research Interests

  • Genetic vaccines
  • Immuno-dermatology: Skin immunology and dendritic cell biology