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Faculty
 
kane.jpg Lawrence P. Kane , Ph.D.
Associate Professor
E1056 BSTWR
200 Lothrop St.
Pittsburgh, PA 15261
Phone: 412.648.8947
Email: lkane@pitt.edu
Fax: 412.383.8096
Faculty Research Interests
 
Research Interests
My lab is currently pursuing several projects:

1. The role of TIM-1 in T cell activation and differentiation
This project currently involves the study of TIM-1, a novel protein of the T cell immunoglobulin and mucin domain family. We have found that TIM-1 can provide a co-stimulatory signal, along with the TCR, that affects T cell activation and differentiation. We are trying to determine what signaling pathways are activated by TIM-1 as well as performing structure/function studies to determine how TIM-1 couples to these pathways.

2. The role of TIM-3 in T cells and dendritic cells
We are also studying the role of another TIM family member - TIM-3 - in regulation of T cell and dendritic cell function. Previous studies have suggested that TIM-3 has different effects on these two cell types, i.e. downgregulation of Th1 T cell function and upregulation of dendritic cell function. Among other things, we are interested in elucidating signaling pathways downstream of TIM-3 in these two cell types.

3. Activation of NF-kB by the Akt kinase
We found that Akt can induce NF-kB-dependent transcription, in cooperation with signals from PKC. We are currently working to identify direct targets of Akt in this pathway, as well as its consequences for T cell activation and CD28 co-stimulation.
 
Education
B.S. - Boston College (1988)

Ph.D. - University of California at San Diego (1996)

Postdoc - University of California at San Francisco (1996-2000)
 
Academic Affiliation
Associate Professor, Department of Immunology, University of Pittsburgh School of Medicine

Director, Immunology Graduate Program
 
Selected Publications
Roth CG, Garner K, Eyck ST, Boyiadzis M, Kane LP, Craig FE. TIM3 expression by leukemic and non-leukemic myeloblasts. Cytometry B Clin Cytom. 2013 May;84(3):167 72. doi: 10.1002/cyto.b.21080. Epub 2013 Mar 29. PubMed PMID: 23554257.

DeFrances MC, Debelius DR, Cheng J, Kane LP. Inhibition of T-cell activation by PIK3IP1. Eur J Immunol. 2012 Oct;42(10):2754-9. doi: 10.1002/eji.201141653. Epub 2012 Jul 19. PubMed PMID: 22706993; PubMed Central PMCID: PMC3654810.

Cheng J, Phong B, Wilson DC, Hirsch R, Kane LP. Akt fine-tunes NF-κB dependent gene expression during T cell activation. J Biol Chem. 2011 Oct 14;286(41):36076-85. doi: 10.1074/jbc.M111.259549. Epub 2011 Aug 23. PubMed PMID: 21862580; PubMed Central PMCID: PMC3195567.

Lee J, Su EW, Zhu C, Hainline S, Phuah J, Moroco JA, Smithgall TE, Kuchroo VK,
Kane LP. Phosphotyrosine-dependent coupling of Tim-3 to T-cell receptor signaling
pathways. Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1. PubMed PMID: 21807895; PubMed Central PMCID: PMC3187355.

Cheng J, Montecalvo A, Kane LP. Regulation of NF-κB induction by TCR/CD28.
Immunol Res. 2011 Aug;50(2-3):113-7. doi: 10.1007/s12026-011-8216-z. Review. PubMed PMID: 21717079.

Su EW, Bi S, Kane LP. Galectin-9 regulates T helper cell function
independently of Tim-3. Glycobiology. 2011 Oct;21(10):1258-65. doi: 10.1093/glycob/cwq214. Epub 2010 Dec 27. PubMed PMID: 21187321; PubMed Central
PMCID: PMC3167474.

Ho AW, Shen F, Conti HR, Patel N, Childs EE, Peterson AC, Hernández-Santos N, Kolls JK, Kane LP, Ouyang W, Gaffen SL. IL-17RC is required for immune signaling via an extended SEF/IL-17R signaling domain in the cytoplasmic tail. J Immunol. 2010 Jul 15;185(2):1063-70. doi: 10.4049/jimmunol.0903739. Epub 2010 Jun 16. PubMed PMID: 20554964; PubMed Central PMCID: PMC2897912.

Kane LP. T cell Ig and mucin domain proteins and immunity. J Immunol. 2010 Mar
15;184(6):2743-9. doi: 10.4049/jimmunol.0902937. Review. PubMed PMID: 20200285; PubMed Central PMCID: PMC3069641.

Hu W, Nessler S, Hemmer B, Eagar TN, Kane LP, Leliveld SR, Müller-Schiffmann
A, Gocke AR, Lovett-Racke A, Ben LH, Hussain RZ, Breil A, Elliott JL, Puttaparthi K, Cravens PD, Singh MP, Petsch B, Stitz L, Racke MK, Korth C, Stüve O. Pharmacological prion protein silencing accelerates central nervous system
autoimmune disease via T cell receptor signalling. Brain. 2010 Feb;133(Pt 2):375-88. doi: 10.1093/brain/awp298. Epub 2010 Feb 9. PubMed PMID: 20145049; PubMed Central PMCID: PMC2822628.

Turner MS, Kane LP, Morel PA. Dominant role of antigen dose in CD4+Foxp3+
regulatory T cell induction and expansion. J Immunol. 2009 Oct 15;183(8):4895-903. doi: 10.4049/jimmunol.0901459. PubMed PMID: 19801514; PubMed Central PMCID: PMC3142864.
 
Grants
  • Title: Regulation of T Cell Activation and Differentiation by TIM-1
    Agency: NIH
    Role: P.I.
    Funding Period: 2007-2012

  • Title: Regulation of NF-κB by the Akt Kinase
    Agency: NIH
    Role: P.I.
    Funding Period: 2007-2011

  • Title: TIM Family of Genes: Role in T Cell Immunity and Tolerance
    Agency: NIH
    Role: Consultant
    Funding Period: 2008-2013
 
Lab Personnel
Graduate Students
Lyndsay Avery
Kristia Hamilton
Binh Phong
Uzodinma Uche

Postdocts
Angela Montecalvo
 
My Links
 
  • Kane Lab Website
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