The Innovative Technologies Development (ITD) Core provides cutting edge gene targeting strategies using various methods, including CRISPR-Cas9 gene targeting. Genetic Engineering in mice using CRISPR/Cas9 technology: Classical transgenesis: Design of transgenic vectors. Assistance with gene editing in cell lines using CRISPR: We offer designs and can provide assistance for genome engineering projects in cell lines. We will advise the investigator on the most effective method to generate their desired cell lines. We will provide the design for a custom targeting strategy, identify targets sites, the design of targeting vectors, cloning procedure, and genotyping strategy. Other/New: The Innovative Technologies Development Core will also consider developing new emerging technologies or other services that would be valuable to investigators of the University of Pittsburgh.
Dr. Sebastien Gingras will consult with the investigator on the design of the targeting strategy. Detailed analysis of the gene structure then provides the best targeting strategy to meet the investigator’s objectives. We provide complete a turnkey service that includes project design, synthesis of sgRNA and Cas9 mRNA, assessment of reagents quality, editing efficiency, and mouse genotyping.
