Instrumentation

Please click on the instrument name for more information about its current configuration.  You can schedule time on any instrument you have been trained to use via ScheduleBook.  To schedule a sort on any of the sorters listed below, please use the Sort Request Form.  Please refer to Core policies on Scheduling and Cancelation. 

When planning new experiments, we recommend using the panel builder app at FluoroFinder (free registration for Pitt users).

If you experience any issues with a machine after hours or on a weekend, please use the Problem Log to report it, and we will resolve the issue as soon as we can.

AURORA

The Auroras are equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).

64 Fluorescence channels:  355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels;  640nm = 8 channels; 561nm = 10 channels.  Efficient spectrum capture for dyes emitting in the 360nm-900nm range.  No filter changes required for any fluorochromes.

Aurora Black has a plate loader for 96 well plates.

Aurora Pink and Aurora Yellow have a plate loader for 96 well plates and a 40 tube holder.

Cytek web page: click here

Aurora tested fluorochromes:  click here

Aurora filter ranges:  click here

AURORA CS

Aurora CS (Cell Sorter)

The Aurora CS is equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).

64 Fluorescence channels:  355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels;  640nm = 8 channels; 561nm = 10 channels.  Efficient spectrum capture for dyes emitting in the 360nm-900nm range.  No filter changes required for any fluorochromes.

6 way sorts into 1.5 ml Eppendorf tubes.  4 way sorts into 5ml FACS tubes.  2 way sorts into 15ml Falcon tubes.  Single Cell sorting into 96 well plates (no other plate sizes at this time)

Cytek web page: click here

Aurora tested fluorochromes:  click here

Aurora filter ranges:  click here

Lasers:  488nm 200mW - 561nm 200mW - 375nm 70mW - 405nm 175mW - 642nm 150mW - 785nm Dark Field Laser

MultiMag 60X,40X,20X

Extended Depth of Field (EDF) 

Auto-Sampler (96 well plates)

12 Imaging Channels

For Configuration, click here.

ARIA-15 3 LASER (was Aria 10)

The Aria is equipped with 3 lasers 488 (blue), 633 (red), 405 (violet), for a total of 10 fluorescent parameters running on Diva 8/Windows 7. For configuration, click here. For Fluorofinder panel design, click here.

FORTESSA ASSEMBLY (was Fortessa 15)

The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

LSR ASSEMBLY (was LSR 10)

The LSR II is equipped with 5 lasers running  on Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 640 100mW Trigon (red).  15 parameters. For configuration, click here.  Fluorofinder panel design, click here.

LSR-15

The LSR II is equipped with 5 lasers running on Diva 8/Windows 7.   Lasers:  355 60mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 633 40mW Trigon (red). 15 Parameters For configuration, click here.  For Fluorofinder panel design, click here.

FORTESSA-7S 

The FORTESSA is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 13 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 20mW Trigon, 561 100mW Octagon, 640 40mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

ARIA-15 HOOD 5 LASER (was Aria 15 Hood)

The Aria is equipped with 5 lasers running Diva 8 on Windows 7.  Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 100mW Trigon, 532 150mW Octagon, 635 30mW Trigon. 15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

Fortessa-12 

The VMI Fortessa is equipped with 4 lasers running Diva 8/Windows 7.  Lasers: 355 20mW Trigon (UV), 405 100mW Octagon (violet), 488 100mW Octagon (blue),  640 100mW Trigon (red). 15 Parameters.  For configuration, click here.  For Fluorofinder panel design, click here.

ARIA ASSEMBLY HOOD (was Aria 10 Hood)

The Rheum Aria is equipped with 5 lasers running Diva 8/Windows 7. Lasers:  355 60mW Trigon (UV), 405 100mW Trigon (Violet), 488 100mW Trigon (Blue), 532 100mW Octagon (Green), 640 100mW Trigon (Red).  15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

ATTUNE

The Attune NxT is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 14 fluorescent parameters, running Windows 7. Lasers: 405 100mW Trigon, 488 100mW Trigon, 561 140mW Octagon, 640 140mW Trigon.  For Fluorofinder panel design, click here.

CANOPY ZELLSCANNER ONE

Canopy's ZellScanner One is a fully intergrated automated imaging system that enables quantitaitve measure of high-plex protein biomarker assays across a range of sample types.  Termed "Chip Cytometry", single cell and whole tissue samples can be loaded onto a chip - the latter via section-on-coverslip attachment.  The enlosed chamber acts as a biorepository, providing antibody staining and washing via stain/wash/photobleach cycles, and then stored with tissue preservation for up to two years.  This non-destructive analysis allows for multiple interrogations of the same sample.